Coding

Part:BBa_K4256006:Design

Designed by: Yuqi Liu   Group: iGEM22_UCAS-China   (2022-09-29)


fcs (feruloyl-CoA synthetase) from Streptomyces sp. V-1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 229
    Illegal NgoMIV site found at 358
    Illegal NgoMIV site found at 362
    Illegal NgoMIV site found at 982
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 205
    Illegal BsaI.rc site found at 541


Design Notes

This enzyme is involved in the first step of the production of vanillin from ferulic acid. Its functions have been characterized in E. coli according to the reference.


Source

Source：Streptomyces sp. V-1 Reference: Yang,W., Tang,H., Ni,J., Wu,Q., Hua,D., Tao,F. and Xu,P. Characterization of two streptomyces enzymes that convert ferulic Acid to vanillin. PLoS ONE 8 (6), E67339 (2013)

References